mouse iga igg double color elispot kit  (Cellular Technology Ltd)

Journal: Human Vaccines & Immunotherapeutics

Article Title: Influenza hemagglutinin and neuraminidase multivalent vaccine elicits broader protective immune responses compared to vaccine formulations composed of hemagglutinin or neuraminidase

doi: 10.1080/21645515.2025.2599611

Figure Lengend Snippet: B cell response after vaccination in naïve or pre-immune mice. DBA/2J mice were vaccinated intranasally at four-week intervals with c-di-AMP as adjuvant. Pre-immune mice were previously infected with seasonal H1N1, H3N2, and IBV. Spleens were collected 7 d after final vaccination for ELISPOT assay to enumerate IgA/IgG antibody secreting cells against individual vaccine components. Statistical differences were analyzed using unpaired t-test with GraphPad Prism 9 software (GraphPad, San Diego, CA, USA). A p value of less than .05 was defined as statistically significant (*, p < .05).

Article Snippet: Spleens were harvested 7 d post final vaccination (n = 3) and ELISPOT assay was performed using Mouse IgA/IgG Double-Color ELISPOT kit (CTL, Shaker Heights, OH, USA) per manufacturer’s instructions.

Techniques: Adjuvant, Infection, Enzyme-linked Immunospot, Software

Journal: NPJ Vaccines

Article Title: Polyvalent mRNA vaccine targeting outer surface protein C affords multi-strain protection against Lyme disease

doi: 10.1038/s41541-025-01326-3

Figure Lengend Snippet: A ELISpot determination of IFN-γ and IL-4 production by draining lymph node cells following 24 h stimulation with an overlapping OspC peptide pool represented by spot-forming units (SFU) per 10 5 lymph node cells. B Flow cytometry analysis of activation-induced markers (AIM) expression (CD25 + OX40+) as a percentage of CD4 + T cells in the dLNs collected 4 weeks after boost vaccination following 18 h stimulation with an overlapping OspC peptide pool. C Flow cytometry analysis of the percentage of CD4+ (top) and CD8+ (bottom) T cells producing IFN-γ, IL-2, or TNF-α. n = 5 per group, error bars represent standard deviation. Statistical significance was calculated using independent two-tailed t -tests. ns not significant, * p value <0.05, ** p value <0.01, *** p value <0.001.

Article Snippet: The ELISpot assay was performed using a Mouse IFN-γ/IL-4 Double-Color ELISPOT kit according to the manufacturer’s instructions (ImmunoSpot by Cellular Technology Limited, Shaker Heights, Ohio, USA).

Techniques: Enzyme-linked Immunospot, Flow Cytometry, Activation Assay, Expressing, Standard Deviation, Two Tailed Test